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3t3-l1 細(xì)胞
細(xì)胞名稱(chēng):3t3-l1 細(xì)胞Ppp2r1b基因敲除株
細(xì)胞簡(jiǎn)稱(chēng):3T3-L1 KO-Ppp2r1b
產(chǎn)品貨號(hào):CGKO-M2175
參考價(jià)格:15187元
修飾基因:Ppp2r1b Gene (ID: 73699)
修飾類(lèi)型:敲除
轉(zhuǎn)導(dǎo)方法:慢病毒轉(zhuǎn)導(dǎo)
抗性基因:Hygro
克隆類(lèi)型:純合子
細(xì)胞鑒定:PCR,測(cè)序
種屬來(lái)源:小鼠
組織來(lái)源:胚胎
細(xì)胞形態(tài):成纖維細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
3t3-l1 細(xì)胞培養(yǎng)體系:DMEM-H+10%CS(新生牛血清)+1%P/S
傳代比例:1:3-1:4,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
3t3-l1 細(xì)胞 是從3T3細(xì)胞(Swissalbino)中經(jīng)克隆分離得到的連續(xù)傳代的亞系。該細(xì)胞從快速分裂到匯合和接觸性抑制狀態(tài)經(jīng)歷了前脂肪細(xì)胞到脂肪樣細(xì)胞的轉(zhuǎn)變。該細(xì)胞鼠痘病毒陰性;可產(chǎn)生甘油三酯,高濃度血清可增強(qiáng)細(xì)胞內(nèi)脂肪堆積.
Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 4426090
Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 1977
Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
Scherer PE, et al. Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93: 131-135, 1996. PubMed: 8552590
Kallen CB, Lazar MA. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 93: 5793-5796, 1996. PubMed: 8650171
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