熱門關(guān)鍵詞: CO2搖床 凝膠成像系統(tǒng) 細胞培養(yǎng)耗材 乳酸分析儀 發(fā)酵罐 Rainin移液槍 振蕩培養(yǎng)箱 生物反應(yīng)器
0512-62956104
PC-12細胞
細胞名稱: 大鼠腎上腺嗜鉻細胞瘤細胞(未分化)
細胞別名:PC-12; PC 12
貨號:TCR-C617
規(guī)格: 1X10^6
價格:1500 元(推薦T25瓶,復蘇發(fā)貨1-2周)
該細胞系來自可移植的雄性大鼠的腎上腺嗜鉻細胞瘤;表達神經(jīng)生長因子(NGF)受體,NGF可誘導該細胞產(chǎn)生可逆性的神經(jīng)表型。該細胞可產(chǎn)生兒茶酚胺、多巴胺和去甲腎上腺素,但不合成腎上腺素。
種屬來源:大鼠
組織來源:腎上腺
疾病特征:嗜鉻細胞瘤
細胞形態(tài):圓形
生長特性:疏松貼壁和懸浮同時存在
培養(yǎng)體系:RPMI-1640+5%FBS+15%馬血清+1%P/S
配套培養(yǎng)基貨號:TCR-G617
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質(zhì)量檢測:細菌、真菌、支原體檢測均為陰性
參考文獻:
Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
當您來到這個頁面時,您已成為我們最尊貴的客戶,蘇州阿爾法生物實驗器材有限公司真誠歡迎您的到來!
0512-62956104
十四年專注于生物實驗室儀器供應(yīng)全國服務(wù)熱線
0512-62956104