HEP-G2人肝癌細(xì)胞

細(xì)胞名稱：HEP-G2人肝癌細(xì)胞

細(xì)胞別稱：HEP-G2; Hep G2; HEP G2; HepG2; HEPG2

細(xì)胞簡(jiǎn)稱：HepG2

產(chǎn)品貨號(hào)：TCH-C196

規(guī)格：1×10?cells/T25 培養(yǎng)瓶

種屬來(lái)源：人

組織來(lái)源：肝

疾病特征：肝癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S

配套培養(yǎng)基貨號(hào)：TCH-G196

傳代比例：1:2-1:4，每2-3天換液一次

傳代周期：72-96 h

培養(yǎng)條件：氣相：95%空氣+5%CO 2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

細(xì)胞來(lái)源：HEP-G2人肝癌細(xì)胞來(lái)源于一名15歲的白人少年的肝癌組織。該細(xì)胞表達(dá)甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、轉(zhuǎn)鐵蛋白、α-1-抗凝乳蛋白酶、結(jié)合珠蛋白、銅藍(lán)蛋白、纖溶酶原、補(bǔ)體C4、C3激活物、纖維蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、視黃醇結(jié)合蛋白；表達(dá)胰島素受體和胰島素樣生長(zhǎng)因子IGFⅡ的受體；該細(xì)胞具有3-羥基-3-甲酰輔酶A還原酶和肝甘油三酯脂肪酶的活性。目前尚未證明 HEP-G2人肝癌細(xì)胞中有HBV基因組。

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