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MRC-5細(xì)胞
細(xì)胞名稱:人胚肺細(xì)胞
細(xì)胞簡稱:MRC-5
產(chǎn)品貨號:TCH-C263
種屬來源:人
組織來源:肺
疾病特征:/
細(xì)胞形態(tài):成纖維細(xì)胞樣
生長特性:貼壁生長
培養(yǎng)體系: DMEM-H+10%FBS+1%P/S
配套培養(yǎng)基貨號:TCH-G263
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質(zhì)量檢測:細(xì)菌、真菌、支原體檢測均為陰性
MRC-5細(xì)胞是一種常用的人類胚肺細(xì)胞,是由1966年從一個正常的男性胎兒的肺部組織獲得,具有非常良好的細(xì)胞生長和增殖特性,可廣泛應(yīng)用于細(xì)胞培養(yǎng)、病毒研究和毒性測試等領(lǐng)域。
該細(xì)胞系來自14周齡男性胎兒的正常肺組織,該細(xì)胞老化前能傳代42~46個倍增時間。
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