熱門關(guān)鍵詞: CO2搖床 凝膠成像系統(tǒng) 細(xì)胞培養(yǎng)耗材 乳酸分析儀 發(fā)酵罐 Rainin移液槍 振蕩培養(yǎng)箱 生物反應(yīng)器
細(xì)胞名稱: 人轉(zhuǎn)移胰腺腺癌細(xì)胞(STR鑒定正確)
細(xì)胞簡(jiǎn)稱:AsPC-1細(xì)胞-人轉(zhuǎn)移胰腺腺癌細(xì)胞
細(xì)胞別名: AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1;
AsPc1貨號(hào):TCH-C128
規(guī)格: 1X10^6
價(jià)格:1200元 (干冰運(yùn)輸需另加200元干冰費(fèi))
AsPC-1細(xì)胞培養(yǎng)基 貨號(hào):TCH-G128
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AsPc-1細(xì)胞-人轉(zhuǎn)移胰腺腺癌細(xì)胞
細(xì)胞名稱: 人轉(zhuǎn)移胰腺腺癌細(xì)胞(STR鑒定正確)
細(xì)胞簡(jiǎn)稱:AsPC-1細(xì)胞
細(xì)胞別名: AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1
貨號(hào):TCH-C128
規(guī)格: 1X10^6
價(jià)格:1200元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來(lái)源:人
組織來(lái)源:胰腺
特征:轉(zhuǎn)移灶;腹水腺癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系:RPMI-1640+10%FBS+1%P/S
AsPC-1細(xì)胞培養(yǎng)基貨號(hào):TCH-G128
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
AsPC-1細(xì)胞 凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
人轉(zhuǎn)移胰腺腺癌細(xì)胞(AsPC-1),取自62歲高加索人女性,該細(xì)胞系是合適的轉(zhuǎn)染宿主。
參考文獻(xiàn):
Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348
Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528
Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226
Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565
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