細胞名稱: c2c12細胞-小鼠成肌細胞
細胞別稱: C2c12; C2-C12; C12
貨號:TCM-C720
規(guī)格: 1X10^6/T25細胞培養(yǎng)瓶
價格:1500元 (干冰運輸需另加200元干冰費 )
c2c12細胞培養(yǎng)基貨號:TCM-G720
c2c12細胞-小鼠成肌細胞 細胞株是YaffeD,SaxelO建立的小鼠成肌細胞系的亞株。
該細胞分化較快,可形成能收縮的微管,產生特異的肌肉蛋白。
在骨形態(tài)形成蛋白(BMP-2)的作用下, c2c12細胞可由成肌細胞分化為成骨細胞。
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c2c12細胞-小鼠成肌細胞
細胞名稱: c2c12細胞-小鼠成肌細胞
細胞別稱: C2c12; C2-C12; C12
貨號:TCM-C720
規(guī)格: 1X10^6/T25細胞培養(yǎng)瓶
價格:1500元 (干冰運輸需另加200元干冰費 )
產品貨號:TCM-C720
種屬來源:小鼠
組織來源:肌肉
細胞形態(tài):成肌細胞樣
生長特性:貼壁生長
培養(yǎng)體系:DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
c2c12細胞-小鼠成肌細胞
配套培養(yǎng)基貨號:TCM-G720
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
c2c12細胞-小鼠成肌細胞 細胞株是YaffeD,SaxelO建立的小鼠成肌細胞系的亞株。該細胞分化較快,可形成能收縮的微管,產生特異的肌肉蛋白。在骨形態(tài)形成蛋白(BMP-2)的作用下, c2c12細胞可由成肌細胞分化為成骨細胞。
參考文獻:
"Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed: 21308772
Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336
Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936
Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064
Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324
Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846
Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524"
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