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CHOK1細(xì)胞
1957年,PuckTT從成年中國倉鼠卵巢的活檢組織建立了CHO細(xì)胞,CHOK1細(xì)胞是CHO的一個(gè)亞克隆。CHOK1細(xì)胞的生長需要脯氨酸。
細(xì)胞名稱:倉鼠卵巢細(xì)胞
細(xì)胞簡稱:CHO-K1
細(xì)胞別稱:CHO K1; CHOK1; CHO cell clone K1; GM15452
產(chǎn)品貨號:TCO-C005
規(guī)格: 1X10^6
種屬來源:倉鼠
組織來源:卵巢
細(xì)胞形態(tài):上皮細(xì)胞樣
生長特性:貼壁生長
培養(yǎng)體系:F12K+10%FBS+1%P/S
配套培養(yǎng)基貨號:TCO-G005
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質(zhì)量檢測:細(xì)菌、真菌、支原體檢測均為陰性
CHOK1細(xì)胞為轉(zhuǎn)化細(xì)胞系, 在工業(yè)生產(chǎn)上應(yīng)用較多, CHOK1細(xì)胞 染色體分布頻率是2n=22,系亞二倍體細(xì)胞。海星細(xì)胞庫保存CHO-K1細(xì)胞株,編號為TCO-C005,被廣泛地用于重組DNA蛋白的表達(dá)。
參考文獻(xiàn):
Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821
Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058
Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736
Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403
Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351
Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752
Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365
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