CHO-K1細(xì)胞-懸浮培養(yǎng)細(xì)胞

    CHO-K1細(xì)胞-懸浮培養(yǎng)細(xì)胞是指將復(fù)蘇CHO-K1細(xì)胞轉(zhuǎn)入T25 cm2細(xì)胞培養(yǎng)瓶，以F12K基礎(chǔ)培養(yǎng)基添加10%血清培養(yǎng)，待細(xì)胞長滿單層后，加入 0.25 % 胰蛋白酶消化，傳代至裝有完全培養(yǎng)基的T25 cm2細(xì)胞培養(yǎng)瓶（Corning）中繼續(xù)培養(yǎng)，當(dāng)細(xì)胞鋪滿瓶底時，即得貼壁 CHO-K1 細(xì)胞。取貼壁CHO-K1 細(xì)胞，換液，加入15 mL含血清的完全基和 15 mL無血清CHO-K1專用培養(yǎng)基，2天后吸出一半培養(yǎng)液，加入等量的無血清CHO-K1，每2天重復(fù)一次此操作，直到胎牛血清濃度低于 1 %后，以基礎(chǔ)培養(yǎng)基B001 繼續(xù)培養(yǎng)，即培養(yǎng)基中血清濃度依次以 5 %，2 .5 %，1.25%，0.625 %，0 %降低。細(xì)胞在無血清CHO-K1專用培養(yǎng)基中密度達(dá)到5 ×106cells/ mL時，即得懸浮 CHO-K1 細(xì)胞。培養(yǎng)條件：傳代培養(yǎng)條件：37±1℃，120rmp，6.0%CO2

細(xì)胞名稱：倉鼠卵巢細(xì)胞（懸?。?

細(xì)胞簡稱：CHO-K1細(xì)胞-懸浮培養(yǎng)細(xì)胞

產(chǎn)品貨號：TCO-C047

種屬來源：倉鼠

組織來源：卵巢

細(xì)胞形態(tài)：圓形

生長特性： 懸浮生長

培養(yǎng)體系：CD CHO Medium+Anti-Clumping Agent+L-Glutamine Solution

配套培養(yǎng)基貨號：TCO-G047

傳代比例：傳代密度：0.2~0.5×106cells/mL；每2-4天傳代一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：94%空氣+6%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

參考文獻(xiàn)：

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