您的位置： 首頁(yè) > 產(chǎn)品頻道 > 實(shí)驗(yàn)室試劑 > 人的elisa試劑盒

人的elisa試劑盒

?顯色時(shí)間25-30分鐘

在線咨詢

全國(guó)熱線

0512-62956104

● 產(chǎn)品概況/ product introduction

貨號(hào)EK0557
產(chǎn)品名稱Human ACE ELISA Kit
產(chǎn)品描述For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.
免疫原NSO, L30-L1261
交叉反應(yīng)There is no detectable cross-reactivity with other relevant proteins.
敏感性＜5pg/ml
檢測(cè)范圍0.78ng/ml-50ng/ml
基因名稱ACE
基因全名angiotensin I converting enzyme
Uniprot IDP12821
指標(biāo)別名ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A
背景資料Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
顯色時(shí)間25-30分鐘

● 試劑盒內(nèi)容/ product introduction

SKU	名稱	數(shù)量
Catalog number	Description	Quantity
EK0557-CAP	96-well plate precoated with anti- human ACE antibody	1
EK0557-ST	Lyophilized recombinant human ACE standard	50ng/tubex2
EK0557-DA	Biotinylated anti- human ACE antibody	130ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4
*小貼士：額外的試劑盒部件可以單獨(dú)購(gòu)買。如需購(gòu)買請(qǐng)和試劑盒一起下單避免額外運(yùn)費(fèi)。

● 需要用戶自己提供的器材和試劑/ others

?96孔讀板儀器。
?自動(dòng)洗板機(jī)。
?移液器和槍頭。建議使用多通道移液器/排槍。
?Eppendorf 管。
?額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內(nèi)提供足量。如需額外緩沖液可參考如下配方)。

● 批內(nèi)差/批間差/ others

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1278	6839	24157	1358	6350	25528
Standard deviation	62.62	465.05	1087.06	88.27	482.6	1225.34
CV(%)	4.90%	6.80%	4.50%	6.50%	7.60%	4.80%

: 尊敬的訪客：

當(dāng)您來(lái)到這個(gè)頁(yè)面時(shí)，您已成為我們最尊貴的客戶，蘇州阿爾法生物實(shí)驗(yàn)器材有限公司真誠(chéng)歡迎您的到來(lái)！

: 聯(lián)系我們
免費(fèi)服務(wù)電話：18934597460
0512-62956104
十四年專注于生物實(shí)驗(yàn)室儀器供應(yīng)

熱品推薦 / Hot product

: ?Solentim ICON細(xì)胞分析儀

SolentimICON是一款集成化的SolentimICON細(xì)胞分析儀，為人IgG滴度、細(xì)胞活力密度（VCD）和產(chǎn)率設(shè)計(jì)。它結(jié)合了STUDIUS數(shù)據(jù)管理平臺(tái)，為生物醫(yī)學(xué)細(xì)胞開(kāi)發(fā)開(kāi)發(fā)行業(yè)的科學(xué)家提供了一個(gè)數(shù)字化的工作流程，幫助他們更快、更早、更安全地做出明智的決策。

: Solentim Cell Metric? X-高內(nèi)涵細(xì)胞成像分析系統(tǒng)-單細(xì)胞成像儀

SolentimCellMetric?X-高內(nèi)涵細(xì)胞成像分析系統(tǒng)-單細(xì)胞成像儀

: Solentim MatriClone? iPSC基質(zhì)膠-單細(xì)胞克隆和生長(zhǎng)促進(jìn)劑

SolentimMatriClone?iPSC基質(zhì)膠-單細(xì)胞克隆和生長(zhǎng)促進(jìn)劑是一種新型的“無(wú)動(dòng)物源”基質(zhì)，旨在支持iPSC的早期生長(zhǎng)，并提高單細(xì)胞鋪板中iPSC的生長(zhǎng)效率。MatriClone優(yōu)化了“in-solution”鋪板法，使之在光學(xué)上更適合自動(dòng)成像，與VIPS鋪板系統(tǒng)結(jié)合使用，可以提高單細(xì)胞的存活及后續(xù)生長(zhǎng)效率，實(shí)現(xiàn)了一種簡(jiǎn)單、自動(dòng)化的工作流程。

: Solentim STUDIUS細(xì)胞系開(kāi)發(fā)設(shè)備

SolentimSTUDIUS細(xì)胞系開(kāi)發(fā)設(shè)備為科學(xué)家提供了一個(gè)一站式的細(xì)胞系開(kāi)發(fā)工作流程，幫助科學(xué)家們更快、更自信地做出決策。STUDIUS整合了SolentimVIPS?/VIPS?PRO單細(xì)胞鋪板、CellMetric?/CellMetric?X克隆驗(yàn)證、克隆生長(zhǎng)監(jiān)測(cè)和ICON?細(xì)胞IgG產(chǎn)率評(píng)估的關(guān)鍵數(shù)據(jù)，提供了一個(gè)新型的決策平臺(tái)。