細胞名稱: 人骨肉瘤細胞(STR鑒定正確)
細胞別稱: U2OS,U-2 OS
細胞簡稱:u2os細胞
貨號:TCH-C365
規(guī)格:1×10?cells/T25 培養(yǎng)瓶
價格:1000元 (干冰運輸需另加200元干冰費)
培養(yǎng)體系:McCoy's 5A+10%FBS+1%P/S
配套培養(yǎng)基貨號:TCH-G365
0512-62956104
u2os細胞
細胞名稱: 人骨肉瘤細胞(STR鑒定正確)
細胞別稱: U2OS,U-2 OS
細胞簡稱:u2os細胞
貨號:TCH-C365
規(guī)格:1×10?cells/T25 培養(yǎng)瓶
價格:1000元 (干冰運輸需另加200元干冰費)
種屬來源:人
組織來源:骨
疾病特征:骨肉瘤
細胞形態(tài):上皮細胞樣
生長特性:貼壁生長
培養(yǎng)體系:McCoy's 5A+10%FBS+1%P/S
配套培養(yǎng)基貨號:TCH-G365
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
u2os細胞 培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
u2os細胞規(guī)格
u2os細胞系(原名2T)是由PontenJ和SakselaE在1964年從一名15歲女孩的脛骨中等分化的肉瘤中分離建立的。與WI-38共培養(yǎng)或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的補體結合試驗(CF法)未檢測到病毒。該細胞表達胰島素樣生長因子Ⅰ、Ⅱ(IGF-Ⅰ、IGF-Ⅱ)受體和骨肉瘤衍生生長因子(ODGF)。
Gao Y, Zhu Y, Wang H, et al. Lipid-mediated phase separation of AGO proteins on the ER controls nascent-peptide ubiquitination[J]. Molecular Cell, 2022, 82(7): 1313-1328. e8.
Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590
Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767
Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487
Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590"
當您來到這個頁面時,您已成為我們最尊貴的客戶,蘇州阿爾法生物實驗器材有限公司真誠歡迎您的到來!
0512-62956104
十四年專注于生物實驗室儀器供應全國服務熱線
0512-62956104